中文名称甲酚紫中文同义词烯酯;甲酚紫;焦油紫,~70%;甲酚紫,高纯生物染色剂;甲酚紫乙酸盐(>70%,BS);甲苯基紫乙酸盐;焦油紫,甲酚紫;CRESYLFASTVIOLET焦油紫英文名称CRESYLVIOLETACETATE英文同义词9-AMINO-5-IMINO-5H-BENZO[A]PHENOXAZINEACETATESALT;CINO51180;CRESYLVIOLET;CRESYLECHTVIOLET;CRESYLECHTVIOLETT;CRESYLVIOLETACETATE;CRESYLVIOLETT;3,7-diaminobenzo[b]phenoxazinyliumacetateCAS号10510-54-0分子式C18H15N3O3分子量321.34EINECS号234-043-3相关类别色素类;生化试剂;标准品;其他生化试剂;有机锂化合物,有机碱;生化试剂-色素类;通用生化试剂-生物染色剂与化学指示剂;PhenoxazineMol文件10510-54-0.mol结构式甲酚紫性质熔点140-143°C(lit.)储存条件roomtemp溶解度水中的溶解度为1mg/mL形态粉末颜色绿色至黑色气味(Odor)无味PH值3(10g/l,H2O,20℃)水溶解性Solubleinwaterandethanol.Insolubleinacetone.最大波长(λmax)596nm,601nm稳定性稳定的。与强氧化剂不相容。生物领域应用Biochemicalandclinicalanalysis;detectingcellularproteins;treatingcardiovasculardiseasesInChIKeyUEWSHLFAYRXNHZ-UHFFFAOYSA-NCAS数据库10510-54-0(CASDataBaseReference)甲酚紫用途与合成方法生物活性CresylVioletacetate是一种红色荧光染色剂,可用于染色神经元。体外研究TheestimatedtotalnumberofSGnChemicalbookeuronsis27,485±3251and26,705±1823inthePVandCresylVioletacetatestainedsections,respectively.Thereisnosignificantdifferencebetweenthem(p=0.552).Therefore,CresylVioletacetatestainingissimplerandmorecosteffectivewhenestimatesneuronalnumber.AlthoughPVstainsspiralganglionneurons(SGNs)withagreaterintensityandprovidesafunctionalstatus,itstediousprotocollimitsitsuseforquantification.TotalRCvolumeisestimatedusingprobeanditisfoundthatanaverageRCvolumeof2.162±0.35mm3and1.82±0.33mm3inCresylVioletacetatestainingandPVimmunostainingsections,respectively.Volumeofneuronsisestimatedusingnucleatorprobeanditis3487.63±951μm3and3740.1±784μm3inCresylVioletacetatestainingandPVimmunostainingsections,respectively.Similarly,volumeofneuronalnucleusisalsoestimatedusingnucleatorprobeanditisfoundtobe131.68±50μm3and126.51±33μm3inCresylVioletacetatestainingandPVimmunostainingsections,respectively. |